Insect Vectors Associated with Viral Diseases of King Chilli (Capsicum chinense Jacq.) in North East India

Authors

  • Rojeet Thangjam College of Horticulture, CAU (Imphal), Thenzawl-796186, Mizoram
  • Veronica Kadam College of PG Studies in Agricultural Sciences, CAU (Imphal), Umiam-793103, Meghalaya
  • P. D. Nath Department of Plant Pathology; Assam Agricultural University, Jorhat- 785 013, Assam
  • R. K . Borah Department of Entomology, Assam Agricultural University, Jorhat- 785 013, Assam

DOI:

https://doi.org/10.55446/IJE.2022.672

Keywords:

Capsicum chinense, viral diseases, CMV, PVY, ChLCV, Aphis gossypii, Myzus persicae, Bemisia tabaci, vectors, transmission, DAS-ELISA, PCR, detection

Abstract

A transmission study was conducted to identify and confirm the insect vectors of viral diseases of king chilli Capsicum chinense Jacquin, at the Virology Laboratory, Assam Agricultural University, Jorhat. The study reveals that Cucumber Mosaic Virus (CMV), Potato Virus Y (PVY) and Chilli Leaf Curl Virus (ChLCV) were the viral diseases transmitted by Aphis gossypii (Glover), Myzus persicae (Sulzer) and Bemisia tabaci (Gennadius), respectively. The detection of CMV and PVY were done through DAS-ELISA assay and ChLCV was done through PCR technique. The results showed that A. gossypii and M. persicae were successfully transmitting the CMV and PVY (40% each). DAS-ELISA revealed that 57.14% of the plant and 66.67% of A. gossypii samples were found to be positive to CMV. While, 42.17 and 60.00 % of PVY detection was observed from plant and M. persicae samples, respectively. B. tabaci also successfully transmitted ChLCV with 80% of the tested plant and the PCR results revealed that the primer pair ChLCV F1 and ChLCV R1 successfully yields 550bp at annealing temperature of 48oC from the infected plant as well as from B. tabaci samples and fail to detect from healthy plants, aphids, and leafhoppers samples.

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Published

2023-09-01

How to Cite

Rojeet Thangjam, Kadam, V., Nath, P. D., & Borah, R. K. . (2023). Insect Vectors Associated with Viral Diseases of King Chilli (<i>Capsicum chinense</i> Jacq.) in North East India. Indian Journal of Entomology, 85(3), 672–675. https://doi.org/10.55446/IJE.2022.672

Issue

Volume 85, Issue 3, September 2023

Section

Research Communications
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Europe PMC

References

Adluri P K, Baldoldiya G M, Nath P D. 2017. Screening of Bhut Jolokia ( Jacq.) germplasm of North East India against Chilli Leaf Curl virus. International Journal of Pure and Applied Bioscience 5 (4): 1189-1196.

Barr N B, Hall D G, Weathersbee A A, Nguyen R, Stansly P, Qureshi J A, Flores D. 2009. Comparison of laboratory colonies and field populations of , an ectoparasitoid of the Asian citrus psyllid, using internal transcribed spacer and cytochrome oxidase subunit I DNA sequences. Molecular Entomology 102 (6): 2325-2330.

Baruah B R, Kashyap A, Nath P D. 2016. Incidence, detection and integrated management of viral disease complex in Bhut Jolokia, a chilli cultivar in Assam. Annals of Plant Protection Sciences 24 (1): 136-141.

Biswas K, Hallan V, Zaidi A A, Pandey P K. 2013. Molecular evidence of Cucumber mosaic virus Subgroup II infecting Capsicum annuum L. in the Western region of India. Current Discovery 2 (2): 97-105.

Butter N S, Rataul H S. 1977. The virus-vector relationship of the tomato leaf curl virus (TLCV) and its vector, Bemisia tabaci gennadius (Hemiptera: aleyrodidae). Phytoparasitica 5: 173.

Clark M F, Adams A N. 1977. Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34 : 475-483.

Garzo E I, Duque M, Fereres A. 2004. Transmission efficiency of different non-persistent viruses infecting melon by four aphid species. Spanish Journal of Agricultural Research 2 (3): 369-376.

Kenganal M Y, Ranebennur H, Byadgi A S. 2006. Management of leaf curl in chilli. The Hindu July. 27.

Kollar A, Seemuller E, Bonnet F, Saillard C, Bove J M. 1990. Isolation of the DNA of various plant pathogenic mycoplasma like organisms from infected plants. Phytopathology 80 (3): 233-237.

Meena R K, Patni V, Arora D K. 2008. Study on phenolics and their oxidative enzyme in Capsicum annuum L. infected with geminivirus. Asian Journal of Experimental Sciences 22(3): 307-310.

Pandey S K, Mathur A C, Srivastava M. 2010. Management of Leaf curl disease of Chilli (Capsicum annuum L.). International Journal of Virology 6 (4): 246-250.

Senanayake D M J B, Verma A, Mandal B. 2006. Virus-vector Relationships, Host range, Detection and Sequence Comparison of Chilli Leaf Curl Virus associated with an epidemic of Leaf curl disease of chilli in Jodhpur. Journal of Phytopathology 160 (3): 146-155.

Shih S L, Tsai W S, Green S K, Singh D. 2006. First Report of Tomato Leaf Curl Joydebpur Virus Infecting Chilli in India. New Disease Reports 14: 17.

Sinha D P, Saxena S, Kumar S, Singh M. 2011. Detection of pepper leaf curl virus through PCR amplification and expression of its coat protein in Escherichia coli for antiserum production. African Journal of Biotechnology 10 (17): 3290-3295.

Talukdar J, Mazumder N, Deka K K, Bora P. 2017. Occurrence of virus diseases of Bhut Jolokia (Capsicum chinense). Indian Journal of Agricultural Research 51 (1): 54-58.

Talukdar J, Saikia A K, Borah P. 2015. Survey and detection of the diseases of Bhut Jolokia (Capsicum chinense Jacq.) in Assam. Journal Crop and Weed 11:186-192.

Thangjam R, Veronica Kadam , Borah R K, Saikia D K, Nath P D, Singh H R. 2021. Effect of IPM modules on major pests and their natural enemies in King chilli Capsicum Chinense in Northeast India. Indian Journal of Entomology. Online published Ref. No. e20420 DoI.: 10.5958/IJE.2021.26.

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