Imidacloprid Induced Reproductive Toxicity in Female Albino Rats


  • Prerna Sood Department of Zoology, Punjab Agricultural University, Ludhiana 141004, Punjab



Imidacloprid, albino rats, ovary, growth, follicles, high dose, low dose, body weight, ovary weight, atretic follicles, healthy follicles, reproduction, cyclicity


Imidacloprid a systemic neonicotinoid, mainly used for controlling insects and pests in agricultural
sector. Two doses of imidacloprid (10 and 20 mg/ kg/ day) were selected based on LD<sub>50</sub> and given through oral intubation to female rats for 60 days. Disturbed cyclicity with significant (P<0.05) decrease in the number of estrous cycle days and increased diestrous index was observed at high dose. Serial sections of ovary were studied for atretic follicles in imidacloprid treated rats. Number of healthy follicles was significantly (P<0.05) decreased with increased number of atretic follicles at high dose of imidacloprid. The histopathology of ovary also revealed more atretic follicles at high dose of imidacloprid. Decreased body weight and ovary weight in imidacloprid treated rats was dose dependent. Based on the observed effects, it can be concluded that imidacloprid produced more significant effects on female reproduction at high dose (20 mg/kgbw/day) as compared to low dose (10 mg/kgbw/day).


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How to Cite

Sood, P. (2023). Imidacloprid Induced Reproductive Toxicity in Female Albino Rats. Indian Journal of Entomology, 85(2), 343–347.



Research Articles


Aitken R J, Koopman P, Lewis S E. 2016. Seeds of concern. Nature 432: 48-52.

Ataya K, Tadros M, Mohammed S. 2008. Cyclophosphamide inhibits antral follicular development in the rat ovary. Biology and Reproduction 38: 157.

Baligar P N, Kaliwal B B. 2002. Induction of gonadal toxicity to female rats after chronic exposure to mancozeb. Indian Health 39: 235-43.

Bhardwaj S, Srivastava M K, Kapoor U, Srivastava L. 2010. A 90 days oral toxicity of IMI in female rats: morphological, biochemical and histophatological evaluations, Food and Chemical Toxicology 48: 1185- 1190.

Borgeest C, Symonds D, Mayer L P, Hoyer P B and Flaws J A. 2002.

Methoxychlor may cause ovarian follicular atresia and proliferation of the ovarian epithelium in the mouse. Toxicological Science 68: 473-78.

Bretveld R W, Thomas C M J, Scheepers P T J, Zielhuis G A and Roeleveld N. 2006. Pesticide exposure: The hormonal function of the female reproductive system disrupted? Reproductive Biology Endocrinology 4: 30-36.

Broznil D, Marinic J, Tota M, Canadi J G and Milin C. 2008. Kinetic evaluation of imidacloprid degradation in mice organs treated with olive oil polyphenol extracts. Crotia Chemical Acta and Reviews 81: 203-09.

Brunet J, Maresca M, Fantini J, Belzunces L P. 2004. Human intestinal absorption of imidacloprid with Caco-2 cells as entercyte model, Toxicological and Applied Pharmacology 194: 1-9.

Budreau C H, Singh R P. 1973. Effect on fenthion and dimethoate on reproduction in the mouse. Toxicology and Applied Pharmacology 26: 29-38.

Chao S and Casida J E. 1997. Interaction of imidacloprid metabolites and analogs with the nicotinic acetylcholine receptor of mouse brain in relation to toxicity. Pesticide Biochemistry and Physiology 58: 77-88.

Cooper R L, Goldman J M, Vanderburgh J G. 1993. Monitering of the estrous cycle in the laboratory rodent by vaginal lavage J.J. Heindel, R.E. Chapin (eds.), Methods in toxicology: Female reproductive toxicology, vol. 3b, Academic press, Sandiego, pp. 45-56

Dawson A H, Eddleston M, Senarathna L, Mohamed F, Gawarammana I, Bowe S J, Manuweera G and Buckley N A. 2010. Acute human lethal toxicity of Agricultural Pesticides: A prospective cohort study. Journal Plos medicine 7: 1000357.

Ding N, Harlow S D, Randolph J F. 2020. Perfluoro alkyl and polyfluoro alkyl substances (PFAS) and their effects on the ovary. Human Reproduction 26: 724-752

Dorrington J H, Chuma A V, Bendell J J. 1988. Transforming growth factor and follicle stimulating hormone promote rat granulosa cell proliferation. Endocrinology 123: 353-59.

Gawade L, Dadarkar S S, Husain R, Gatne M. 2013. A detailed study of developmental immunotoxicity of imidacloprid in Wistar rats. Food and Chemical Toxicology 51: 61-70.

Gill S A, Rizvi F, Khan M Z, Khan A. 2011. Toxic effects of cypermethrin and methamidophos on bovine corpus luteal cells and progesterone production. Experimental and Toxicologic pathology 63(1): 131-5.

Gonsioroski A, Mourikes V E, Flaws J A. 2020. Endocrine disruptors in water and their effects on the reproductive system. International Journal of Molecular Sciences 21: 1929

Guney M, Baha O, Hilmi D, Gulnur T, Seren G G, Irfan A and Tamer M. 2007a. Fallopian damage induced by organophosphate insecticide methyl parathion, and protective effect of vitamins E and C on ultrastructural changes in rats. Toxicology and Indian Health 23: 429-38.

Güney M, Hilmi D, Baha O, Meltem O, Gokhan B and Irfan A. 2007b. Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C. Human and Experimental Toxicology 26: 491-98.

Gupta R K, Miller K P, Babus J K and Flaws J A. 2006. Methoxychlor inhibits growth and induces atresia of antral follicles through an oxidative stress pathway. Toxicological Sciences 93: 382-9.

Holmstrup M, Bindesb A, Oostingh G, Dusch A, Scheil V, Köhler H, Loureiro S, Soares A, Ferreira A, Kienle C, Gerhardt A, Laskowski R, Kramarz P, Bayley M, Svendsen C, Spurgeon D. 2010. Interactions between effects of environmental chemicals and natural stressors: A review, Science and Total Environment 408: 3746-3762.

Jadaramkunti U C, Kaliwal B B. 2019. Effect of dicofol formulation on estrous cycle and follicular dynamicsin albino rats. Journal of Basic and Clinical Physiology and Pharmacology10: 305-19.

Kaur S and Dhanju C K. 2005. Biochemical effects of some organophosphorus pesticides on the ovaries of albino rats. Indian Journal of Physiology and Pharmacology 49: 148-52

Kaur P K, Guraya, S S. 2003. Follicular growth and kinetics during the estrous cycle, pregnancy and postpartum in the Indian mole rat (Bandicota bengalensis) American Journal of Anatomy 166: 469-82.

Lonare M, Kumar M, Raut S, Badgujar P, Doltade S, Telang A. 2014. Evaluation of imidacloprid-induced neurotoxicity in male rats: A protective effect of curcumin. Neurological Chemical International 78: 122-129.

Martinez E M, Swartz W J. 1991. Effect of methoxychloron the reproductive system of the adult female mouse. Gross and histological observations. Reproductive Toxicology 5: 139-147.

Math J R, Jadaramkunti U C, Kaliwal B B. 1998. Effect of edifenphos on follicular dynamics in albino rats. Indian Journal of Experimental Biology 36: 39-42.

McLachlan J A. 2001. Environmental signalling: what embryos and evolution teach us about endocrine disrupting chemicals? Endocrine Reviews 22: 319-41.

Muthviveganandavel V, Muthuraman P, Muthu S and Srikumar K. 2008. A study on low dose cypermethrin induced histopathology, lipid peroxidation and marker enzyme changes in male rat. Pesticide Biochemistry and Physiology 9: 12-16.

Nada M H. Al-Hamdani, Yajurvedi H N. 2017. Effect of cypermethrin on the ovarian activity and its impact on fertility and pubertal onset of offspring, Beni-Suef University Journal of Basic and Applied Sciences 6(4): 374-382

Pederson T, Peter H. 1968. Proposal for a classification of oocytes and follicles in the mouse ovary Journal of Reproduction and Fertility 17: 555-557.

Pernot G A, Saci S, Kernanec P Y. 2017. Embryonic exposure to the widely-used herbicide atrazine disrupts meiosis and normal follicle formation in female mice. Science and Reproduction 7: 3526.

Plowchalk D R, Smith B J, Mattison D R. 1993. Assessment of toxicity to the ovary using follicle quantiation and morphometrics. In: Methods in toxicology: female reproductive toxicology, eds. by Heindel J J, Chapin R E, Vol.3 B, 57-8, Academic Press, San Diego.

Proenca, P, Teixeira H, Castanheira F, Pinheiro J, Monsanto P V, Marques E P and Vieira D N. 2005. Two fatal intoxication cases with imidacloprid: LC/ MS analysis. The non significant changes in the values of BW Forensic Science International 153: 75-80.

Radhika M P and Kaliwal B B. 2002. Monocrotophos induced dysfunction of estrous cycle and follicular development in mice. Indian Health 40: 237-24.

Schulz-Jander D A, Casida J E. 2002. Imidacloprid insecticide metabolism: human cytochrome P450 isozymes differ in selectivity for imidazolidine oxidation versus nitroimine reduction. Toxicology Letters 132: 65-70.

Soratur S M, Kaliwal B B. 2000. Effect of methyl parathion formulation on estrous cycle and reproductive performance in albino rats. Indian Journal of Experimental Biology 37: 176-8.

Stoker T E, Goldman J M, Cooper R L. 2003. The dithiocarbamate fungicide thiram disrupts the hormonal control of ovulation in the female rat. Reproductive Toxicology 7: 211-8.

Vohra P and Khera K S. 2016. Effect of Imidacloprid on Reproduction of Female Albino Rats in Three Generation Study Journal of Veterinary Science and Technology 7(4): 2-7.

Vohra P and Khera K S. 2018. Imidacloprid induced toxicity in ovary of female wistar rats in two generations. Applied Biological research 20(1): 62-67.

Whitehorn P R, O’Connor S, Wackers F L, Goulson D. 2012. Neonicotinoid pesticide reduces bumble bee colony growth and queen production Science 336: 351-352.

Yang E C, Chuang Y C, Chen Y L, Chang L H. 2008. Abnormal foraging behaviour induced by sublethal dosage of imidacloprid in the honey bee (Hymenoptera: Apidae), Journal of Economics Entomology 101: 1743- 1748.

Yavasoglu A, Sayim F, Cyanikgil Y, Turgut M and Yavasoglu N. 2006. The pyrethyroid cypermethrin induced biochemical and histological alterations in rat liver, Journal of Health Science 52: 774-780.