Morphological and Molecular Characterization of Forensically Important Blow Fly Chrysomya megacephala (F.)


  • Nirupama Singh Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan
  • N. P. Singh Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan
  • Vinod Kumari Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan
  • Vikas Jindal Insect Molecular Biology Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana 147021, Punjab
  • Shashi Meena Department of Zoology, University of Rajasthan, Jaipur 302004, Rajasthan



Chrysomya megacephala, Diptera, morphology, adult, maggots, DNA barcoding, BLAST, ClustalW, phylogenetic analysis


Considering the significance of Chrysomya megacephala (F.) (Diptera: Calliphoridae) and their larval instars in forensic-entomological investigations, a culture of C. megacephala was established and morphological features of second and third larval instars were studied under a compound microscope. To validate the species’ morphological identification, sequencing of the ‘standard barcode region’ (658 base pairs of mitochondrial cytochrome oxidase subunit I gene) was performed and this sequence was subjected to BLAST analysis using the NCBI database which acknowledged it as Chrysomya megacephala with 99.39% identity with sequence of same species collected from Maharashtra, India. The phylogenetic analysis congregated Jaipur population separately than other samples from different regions of India, however, not much variations were recorded. This is the maiden report of molecular identification of C. megacephala from Rajasthan.


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How to Cite

Singh, N. ., Singh, N. P. ., Kumari, V., Jindal, V. ., & Meena, S. . (2022). Morphological and Molecular Characterization of Forensically Important Blow Fly <i>Chrysomya megacephala</i> (F.). Indian Journal of Entomology, 84(2), 301–306.



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