Management of Borer and Flubendiamide Residue in Okra Using Milk-Made Bioagent and Calcium Hypochlorite


  • K. Elakkiya Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu
  • P. Yasodha Department of Plant Protection, Anbil Dharmalingam Agricultural College and Research Institute (TNAU), Tiruchirappalli 620027, Tamil Nadu
  • C. Gailce Leo Justin Department of Plant Protection, Anbil Dharmalingam Agricultural College and Research Institute (TNAU), Tiruchirappalli 620027, Tamil Nadu



Okra, Shoot and Fruit Borer, Earias spp., Imidacloprid, Milkoid, Bleaching Powder, Lactic Acid Bacterial Formulation.


Okra is an important vegetable, and it is subject to heavy yield losses by insect pests, of which the shoot and fruit borer Earias spp. is serious. Insecticides are used against this pest, leading to insecticide residues. This study evaluates Milkoid, an LAB formulation spray in field by spraying in tandem with flubendiamide and bleaching powder (calcium hypochlorite). The results indicate that the population of epiphytic LAB density was maximum on plants sprayed with Milkoid with or without flubendiamide. The pest damage was significantly reduced after spraying flubendiamide with or without bleaching powder (3.76- 5.30%). Milkoid accelerated the degradation of flubendiamide with the dissipation rate of 70.07% on 15th day after spray. The efficacy of Milkoid in reducing the toxicity and the potential of calcium hypochlorite as an antimicrobial agent and the shoot and fruit borer management are discussed.


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How to Cite

Elakkiya, K. ., Yasodha, P., & Justin, C. G. L. (2022). Management of Borer and Flubendiamide Residue in Okra Using Milk-Made Bioagent and Calcium Hypochlorite. Indian Journal of Entomology, 84(2), 466–469.



Research Communications


Aktar W, Sengupta D, Chowdhury A. 2009. Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary Toxicology 2(1): 1-12.

Cho K M, Math R K, Islam S M A, Lim W J, Hong S Y, Kim J M, Yun M G, Cho J J, Yun H D. 2009. Biodegradation of chlorpyrifos by lactic acid bacteria during kimchi fermentation. Journal of Agricultural and Food Chemistry 57(5): 1882-1889.

David P M M, Mathialagan M, Prabina J J, Sabarinathan K G, Pushpam A K, Elanchezhyan K, Edward Y S J T, Mohankumar S. 2018. Honey-simulated discriminate fermentation process for preserving perishable juices as probiotics without chemical preservatives. Patent Application 201841016405 dated 1.5.2018 (Trade name: TNAU Winectar)

Elakkiya K, Yasodha P, Justin C G L, Ejilane J, Somasundaram S, David P M M. 2019. Suppression of sucking insects in okra after spraying imidacloprid and milk-made lactic acid bacterial formulation. The Pharma Innovation Journal 8(6): 592-598.

Farag R S, Latif A, El-Gawad A E A, Dogheim S M. 2011. Monitoring of pesticide residues in some Egyptian herbs, fruits and vegetables. International Food Research Journal 18(2).

Gemede H F, Ratta N, Haki G D, Woldegiorgis W Z, Beyene F. 2015. Nutritional quality and health benefits of okra (Abelmoschus esculentus): a review. Journal of Food Processing Technology 25(1): 16-25.

Green J. 2000. Adjuvant outlook for pesticides. Pesticide Outlook 11(5): 196-199.

Hamid F, Hamid F H. 2015. Manual of methods of analysis of foods. Food safety and standards authority of India. 256 pp.

Hussain S, Hartley C J, Shettigar M, Pandey G. 2016. Bacterial biodegradation of neonicotinoid pesticides in soil and water systems. Microbiology Letters 363(23).

Islam S, Asraful M, Math R K, Cho K M, Lim W J, Hong S Y, Kim J M, Yun M G, Cho J J, Yun H D. 2010. Organophosphorus hydrolase (OpdB) of Lactobacillus brevis WCP902 from kimchi is able to degrade organophosphorus pesticides. Journal of Agricultural and Food Chemistry 58(9): 5380-5386.

Lamont J R, Wilkins O, Bywater-Ekegard M, Smith D L. 2017. From yogurt to yield: Potential applications of lactic acid bacteria in plant production. Soil Biology and Biochemistry 111: 1-9.

Russell A D. 1990. Bacterial spores and chemical sporicidal agents. Clinical microbiology reviews 3(2): 99-119.

Srinivasnaik S, Kuttalam S, Philip H, Bhuvaneswari K. 2015. Study on pesticide usage pattern by the farmers in major okra growing areas of Coimbatore district in Tamil Nadu. International Conference on innovative insect management approaches for sustainable agro ecosystem. Madurai, Tamil Nadu.

Sugimoto S, Sonomoto V. 2008. Molecular chaperones in lactic acid bacteria: physiological consequences and biochemical properties. Journal of Bioscience and Bioengineering 106(4): 324-336.

Trivedi P, Sharma V P, Srivastava L P, Malik S. 2014. Multiresidue analysis of organophosphorus pesticides in fruits and vegetables by GC-NPD. International Journal of Advanced Research 2 (10): 600-606.

Wang D, Wenjun L, Yan R, Liangliang D, Donglei Z, Yanrong Y, Qiuhua B, Heping Z, Bilige M. 2016. Isolation and identification of lactic acid bacteria from traditional dairy products in Baotou and Bayannur of midwestern Inner Mongolia and q-PCR analysis of predominant species. Korean Journal for Food Science of Animal Resources 36(4): 499.

Zhou X, Zhao X. 2015. Susceptibility of nine organophosphorus pesticides in skimmed milk towards inoculated lactic acid bacteria and yogurt starters. Journal of the Science of Food and Agriculture 95(2): 260-266.

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