Methods of Extraction of Mucin From Giant African Snail Achatina fulica Bowdich


  • Partha Pratim Gyanudoy Das Department of Entomology, Assam Agricultural University, Jorhat 785013, Assam
  • Badal Bhattacharyya Department of Entomology, Assam Agricultural University, Jorhat 785013, Assam
  • Sudhansu Bhagawati Department of Entomology, Assam Agricultural University, Jorhat 785013, Assam
  • Dhruba Jyoti Nath Department of Soil Science, Assam Agricultural University, Jorhat 785013, Assam
  • Kritideepan Sarmah Department of Biochemistry and Agricultural Chemistry, Assam Agricultural University, Jorhat 785013, Assam



Achatina fulica, mucin, terrestrial mollusc, pharmaceutical, solvent, mechanical methods, gastropod pest, dichloromethane, smoking method, rough tiles, acharan sulfate


Achatina fulica Bowdich is one of the most notorious pests in crop fields. However, the mucin secreted by this terrestrial mollusc has magnificent pharmaceutical attributes. In the present study, solvent and mechanical methods were explored to find out the best method of mucin extraction from this gastropod pest under laboratory conditions. In case of both medium and large sized snails, dichloromethane registered the maximum (2.79 ml and 2.94 ml, respectively) mucin collection out of the six solvents tested. Volume wise comparison showed that the maximum amount of mucin recovery was possible in the highest volume (3 ml) of solvents applied for both the age groups. Of all the mechanical methods tested, smoking method yielded maximum (2.05 ml and 3.02 ml) amount of mucin from both medium and large sized snails, respectively. However, the minimum mucin recovery was registered when the snails were allowed to move over rough tiles.


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How to Cite

Das, P. P. G. ., Bhattacharyya, B. ., Bhagawati, S., Nath, D. J. ., & Sarmah, K. . (2022). Methods of Extraction of Mucin From Giant African Snail <i>Achatina fulica</i> Bowdich. Indian Journal of Entomology, 84(2), 296–300.



Research Articles


Adikwu M U, Okafor J O. 2006. Evaluation of mucin as a release enhancer for rectal delivery of glibenclamide. Current Drug Delivery 3: 243-254.

Adikwu M U. 2005. Evaluation of snail mucin motifs as rectal absorption enhancer for insulin in non-diabetic rat model. Biological and Pharmaceutical Bulletin 28(9): 1801-1804.

Babalola O O, Akinsaoyinu A O. 2009. Proximate composition and mineral profile of snail meat from different breeds of land snail in Nigeria. Pakistan Journal of Nutrition 8(12): 1842-1844.

Campion M. 1961. The structure and function of the cutaneous glands in Helix aspersa. Quarterly Journal of Microscopical Science 102: 195-216.

Ehara T, Kitajima S, Kanzawa N, Tamiya T, Tsuchiya T. 2002. Antimicrobial action of achacin is mediated by L-amino acid oxidase activity. FEBS Letters 531: 509-512.

Gabriel U I, Mirela S, Ionel J. 2011. Quantification of mucoproteins (Glycoproteins) from snail Mucus, Helix aspersa and Helix pomatia. Journal of Agroalimentary Processess and Technologies 17(4): 410-413.

Greistorfer S, Klepal W, Cyran N, Gugumuck A, Rudoll L, Suppan J, Von Byern J. 2017. Snail mucus-glandular origin and composition in Helix pomatia. Zoology 122: 126-138.

Harti A S, Sulisetyawati S D, Murharyati A, Oktariani M. 2016. The effectiveness of snail slime and Chitosan in wound healing. International Journal of Pharma Medicine and Biological Sciences 5(1): 76-80.

IBM Corp. 2012. IBM SPSS statistics for windows, version 21.0. Armonk, New York, USA.

Iguchi M M, Aikawa T, Mastsumoto J J. 1982. Antibacterial activity of snail mucus mucin. Comparative Biochemistry and Physiology 72(3): 571-574.

Jeong J, Toida T, Muneta Y, Kosiishi I, Imanari T, Linhardt R J, Choi H S, Wu S J, Kim Y S. 2001. Localization and characterization of acharan sulphate in the body of the Giant African Snail, Achatina fulica. Comparative Biochemistry and Physiology 130: 513-519.

Kim K J, Chang I M, Toida T, Park Y, Linhardt R J. 1996. A new glycosaminoglycan from the Giant African snail, Achatina fulica. The Journal of Biological Chemistry 271(20): 11750-11755.

Lee Y S, Yang H O, Shin K H, Choi H S, Jung S H, Kim Y M, Oh D K, Linhardt R J, Kim Y S. 2003. Suppression of tumour growth by a new glycosamionoglycan isolated from the African giant snail, Achatina fulica. European Journal of Pharmacology 465: 191-198.

Lowe S, Browne M, Boudjelas S, De Poorter M. 2000. 100 of the world’s worst invasive alien species: a selection from the global invasive species database. Invasive Species Specialist Group (ISSG), a specialist group of the Species Survival Commission (SSC) of the World Conservation Union (IUCN), University of Auckland, New Zealand.

Raut S K, Barker G M. 2002. Achatina fulica Bowdich and other Achatinidae as pests in tropical agriculture. Barker G M (ed.). Molluscs as crop pest. CABI International, Wallingford, UK. pp. 55-114.

Simkiss K, Wilbur K. 1977. The molluscan epidermis and its secretions. Symposia of the Zoological Society of London 39: 35-76.

Swapna P, Reddy T R. 2015. Quantification of mucoproteins (glycoproteins) from slugs mucus, Arion hortensis and Arion ater. International Journal of Pharmaceutical Research and Bio-Science 4(2): 242-250.

Vieira T C R G, Costa-Filho A, Salgado N C, Allodi S, Valente A P, Nasciutti L E, Silva L C F. 2004. Acharan sulfate, the new Glycosaminoglycan from Achatina fulica Bowdich 1822: Structural heterogeneity, metabolic labelling and localization in the body, mucus and organic shell matrix. European Journal of Biochemistry 271: 845-854.