Identification and Characterization of Mosquitocidal Toxins From Bacillus cereus VCRC-641

Authors

  • S. Manikandan Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • K. Aneha Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Bhagyashree Bora Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • V. Abhisubesh Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • P. Hemaladkshmi Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Kakhuangailiu Gangmei Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Sahadiya Mandodan Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Jibi Lukose Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • A. Mathivanan Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • K. Vijayalakshmi Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • S. Poopathi Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

DOI:

https://doi.org/10.55446/IJE.2023.834

Keywords:

Bacillus cereus, native page, LC-MS, SDS-PAGE, uncharacterized protein, amino acids, Trypsin, mosquitocidal, toxins, vector control, bio assay, Clarias batrachus

Abstract

Bacillus cereus VCRC 641 was found to be an alternate larvicidal bacterial agent which was newly isolated from fresh water fish, Clarias batrachus (walking cat fish). The protein responsible for mosquito toxic effect was separated and purified by Sephacryl S-200 column chromatography and NATIVE PAGE. It was observed that a unique protein with molecular weight of 70kDa was the responsible factor for mosquito killing effect. Further analysis of the protein by liquid chromatography-mass spectrometry (LC-MS) it was identified as “Uncharacterized Protein”. The length of the uncharacterized protein was around 158bp. The uncharacterized protein was expected to have a molecular mass of 70 kDa, 51 amino acids, confidence score of 78.45 and mass of 206772.57. Therefore, in the present study we report for the first time that uncharacterized protein (70kDa) was the responsible factor for the mosquito killing effect.

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Author Biographies

K. Aneha, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. K. Aneha,

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Bhagyashree Bora, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Bhagyashree Bora

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

V. Abhisubesh, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Mr. V. Abhisubesh

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

P. Hemaladkshmi, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. P. Hemaladkshmi

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Kakhuangailiu Gangmei, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Kakhuangailiu Gangmei

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Sahadiya Mandodan, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Sahadiya Mandodan

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Jibi Lukose, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Jibi Lukose

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

A. Mathivanan, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Mr. A. Mathivanan,

Technical Officer-B,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

K. Vijayalakshmi, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Mrs. K. Vijayalakshmi

Technical Officer-C,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

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Published

2023-03-23

How to Cite

Manikandan, S., Aneha, K., Bora, B., Abhisubesh, V., Hemaladkshmi, P., Gangmei, K., … Poopathi, S. (2023). Identification and Characterization of Mosquitocidal Toxins From <i>Bacillus cereus</i> VCRC-641. Indian Journal of Entomology, 86(2), 543–546. https://doi.org/10.55446/IJE.2023.834

Issue

Section

Research Communications

References

Allievi M C, Palomino M M, Prado Acosta M, Lanati L, Ruzal S M, Sanchez-Rivas C. 2014. Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus. PLoS One 9(10): 111-114.

Charles J F, Nielsen-LeRoux C. 2000. Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena. Memorias Do Instituto Oswaldo Cruz 95: 201-206.

Federici B A, Park H W, Sakano Y. 2006. Insecticidal protein crystals of Bacillus thuringiensis. In: Shively JM, editor. Microbiology monographs series, inclusions in prokaryotes. Berlin: Springer 195-236.

Haynes S R, Hudson M, Park H W. 2011. Isolation of novel Bacillus species showing high mosquitocidal activity against several mosquito species. Journal of Invertebrate Pathology 107: 79-81.

Hofte H, Whiteley H. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews 53(2): 242-255.

Mustafa M S, Rasotgi V, Jain S, Gupta V J M J A F I. 2015. Discovery of fifth serotype of dengue virus (DENV-5): A new public health dilemma in dengue control. Medical journal armed forces India 71(1): 67-70.

Normile D. 2013. Surprising new dengue virus throws a spanner in disease control efforts. Science 342(6157): 415.

Oei C, Hindley J, Berry C. 1992. Binding of purified Bacillus sphaericus binary toxin and its deletion derivatives to Culex quinquefasciatus gut: elucidation of functional binding domains. Microbiology 138(7): 1515-1526.

Poopathi S, Abidha S. 2009. A medium for the production of biopesticides (Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis) in mosquito control. Journal of Economic Entomology 102(4): 1423-1430.

Poopathi S, Archana B. 2012. A novel cost-effective medium for the production of Bacillus thuringiensis subsp. israelensis for mosquito control. Tropical Biomedicine 29(1): 81-91.

Poopathi S, Tyagi B K. 2002. Studies on Bacillus sphaericus toxicity-related resistance development and biology in the filariasis vector, Culex quinquefasciatus (Diptera: Culicidae) from South India. Applied Entomology and Zoology 37(3): 365-371.

Poopathi S, Thirugnanasambantham K, Mani C, Ragul K, Sundarapandian S M. 2014. Isolation of mosquitocidal bacteria (Bacillus thuringiensis, B. sphaericus and B. cereus) from excreta of arid birds. Indian Journal of Experimental Biology 52: 739-747.

Wirth M C, Walton W E, Federici B A. 2010. Evolution of resistance to the Bacillus sphaericus Bin toxin is phenotypically masked by combination with the mosquitocidal proteins of Bacillus thuringiensis subspecies israelensis. Environmental Microbiology 12(5): 1154-1160.

World Health Organization. 2014. Vector borne diseases. Fact sheet 2014. http://www.who.int/mediacentre/factsheets/fs387/en/.

World Health Organization. 2018. World malaria report 2018. http://apps.who.int/iris/bitstream/handle/10665/275867/9789241565653.

World Health Organization. 2021. Dengue / Fact sheet 2021. https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue

World Health Organization. 2021. Lymphatic filariasis / Fact sheet 2021. https://www.who.int/news-room/fact-sheets/detail/lymphatic-filariasis.

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