Characterization of Dominant Cuticular Hydrocarbons in Inversion and Inversion-Free Strains of Drosophila ananassae (Doleschall)


  • Kavitha R. S. Department of Zoology, Teresian College
  • Jayaramu S. C. Department of Zoology, Yuvaraja’s College, Mysuru 570011, Karnataka



Drosophila ananassae, alkenes, CHCs, chromosomal inversion, chemical cues, desiccation resistance, GC-MS, inversion free, linear alkanes, methylated alkanes, length variation, carbon chain length


Cuticular hydrocarbons (CHCs) of Drosophila ananassae (Doleschall) was characterized and identified using gas chromatography and mass spectrometry (GC-MS) analysis. A high % of methyl-branched alkanes were identified in all inversion (2LA, 3LA and 2LA+3LA) and inversion-free strains followed by linear alkanes and alkenes. The present work unfolds the significant patterns of variations in the isomeric forms of methylated alkanes between the inversion and inversion free strains (F-46.6; df-3, p 0.005), and non-significant between the sex (F-2.14; df-1, p 0.2394). But in linear alkanes shows significant variation between the inversion strains (F-30.49; df-3, p 0.009) and between the male and female (F-115.45; df-1, p 0.001) was observed. In particular there is a significant correlation between the chromosomal inversion and synthesis of CHCs in D. ananassae. Unique blend of CHCs in Drosophila performs dual role as desiccation resistance and act as chemical signalling molecule. Linear alkanes are majorly involved in desiccation resistance but in methyl- branched CHCs length variation is a key determinant of desiccation resistance. Presence of longer methyl- branched alkanes and higher desiccation resistance, shorter the carbon chain length act as a signalling molecules. The current study revealed the influence of chromosomal inversion on the cuticular hydrocarbon profile in D. ananassae.


Download data is not yet available.


Metrics Loading ...




How to Cite

R. S., K., & S. C., J. (2024). Characterization of Dominant Cuticular Hydrocarbons in Inversion and Inversion-Free Strains of <i>Drosophila ananassae</i> (Doleschall). Indian Journal of Entomology, 86(2), 440–444.



Research Articles


Ashburner M. 1989. Drosophila. A laboratory hand book. Cold spring harbor laboratory press 31: 356-76.

Blomquist G J, Ginzel M D. 2021. Chemical ecology, biochemistry, and molecular biology of insect hydrocarbons. Annual Review of Entomology 66: 45-60. DOI:

Chung H, Loehlin D W, Dufour H D, Vaccarro K, Millar J. G, Carroll S B. 2014. A single gene affects both ecological divergence and mate choice in Drosophila. Science 343(6175): 1148-1151. DOI:

Etges W J, Jackson L L. 2001. Epicuticular hydrocarbon variation in Drosophila mojavensis cluster species. Journal of Chemical Ecology 27: 2125-2149. DOI:

Hegde S N. 1999. Studies on the occurrences and distribution of Drosophila species of melanogaster species group in certain parts of south India. Thesis submitted to University of Mysore.

Holze H, Schrader L, Buellesbach J. 2021. Advances in deciphering the genetic basis of insect cuticular hydrocarbon biosynthesis and variation. Heredity 126(2): 219-234. DOI:

Hatano E, Wada-Katsumata A, Schal C. 2019. Environmental decomposition of cuticular hydrocarbons generates a volatile pheromone that guides insect social behaviour. bioRxiv:773937. DOI:

Jackson L L, Bartelt R J. 1986. Cuticular hydrocarbons of Drosophila virilis: comparison by age and sex. Insect Biochemistry 16(2): 433-439. DOI:

Jayaramu S C. 2009. Influence of inversion karyotype on isozyme variants, sexual behaviour and morphophenotypic traits of D. ananassae. Thesis: University of Mysore. pp. 1-169.

Lockey K H. 1988. Lipids of the insect cuticle: origin, composition and function. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 89(4): 595-645. DOI:

Pardy J A, Rundle H D, Bernards M A, Moehring A J. 2019. The genetic basis of female pheromone differences between Drosophila melanogaster and D. simulans. Heredity 122(1): 93-109. DOI:

Sturtevant A H. 1926. The North American species of Drosophila. Carnegie Institution of Washington publication 301: 1-150.

Wang Z, Receveur J P, Pu J, Cong H, Richards C, Liang M, Chung H. 2022. Desiccation resistance differences in Drosophila species can be largely explained by variations in cuticular hydrocarbons. eLife 11: e80859. DOI:

Wilkes H. (Ed.). 2020. Hydrocarbons, oils and lipids: Diversity, origin, chemistry and fate. Cham, Switzerland. Springer International Publishing: 1-46. DOI: