Survival and Virulence of Native Strains of Steinernema carpocapsae and Heterorhabditis bacteriophora in Formulations

Authors

  • Seenivasan Nagachandrabose Department of Nematology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu

DOI:

https://doi.org/10.55446/IJE.2021.30

Keywords:

Earias vittella, Steinernema carpocapsae, Heterorhabditis bacteriophora, formulations, alginate gel, talc, sponge and water concentrates, survival, virulence, storage temperature, viability, infectivity

Abstract

In this study, entomopathogenic nematodes (EPN) isolated from cotton ecosystem viz., Steinernema carpocapsae (strain APKS2) and Heterorhabditis bacteriophora (strain KKMH1) were evaluated in formulations of alginate gel, talc, sponge and water concentrates. The survival or longevity of infective juveniles (IJs) in these formulations was evaluated under in vitro at storage temperatures of 5 and 25 °C. Simultaneously the virulence of these stored EPN infective juveniles (IJs) was evaluated against the spotted boll worm Earias vitella through in vitro bioassays. The results revealed that S. carpocapsae (APKS2) and H. bacteriophora (KKMH1) formulated in alginate gel survived 70-100% up to 3 months with 55-100% infectivity at 5°C and survived 52-100% with 42.5-100% virulence for 2.5 months at 25°C. In talc formulation, these EPN remained alive to an extent of 55-100% with 70-100% virulence at 5°C and stayed alive 45-100% with 52.5-100% virulence at 25°C for 2 months. In sponge formulation, EPN strains survived 55-100% with 67.5-100% infectivity for 7 weeks at 5°C, but with 50-100% survivals and 62.5-100% virulence at 25°C. EPN strain S. carpocapsae (APKS2) can be stored in water at 5°C up to 8 weeks with viability of 50% and infectivity 60%. At 25°C, it can be stored for 5 weeks with survival of 62% and infectivity of 62.5-100 %. It is inferred from the results that alginate and talc formulations at storage temperature of 5°C is better for long term storage of S. carpocapsae strain APKS2 and H. bacteriophora strain KKMH1. Thus, the results revealed that S. carpocapsae (strain APKS2) and H. bacteriophora (strain KKMH1) can be formulated in sponges for use within 2 months and can be kept as water concentrate if they would be utilized in 2-3 weeks.

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Published

2021-09-30

How to Cite

Nagachandrabose, S. . (2021). Survival and Virulence of Native Strains of <i>Steinernema carpocapsae</i> and <i>Heterorhabditis bacteriophora</i> in Formulations. Indian Journal of Entomology, 84(1), 29–33. https://doi.org/10.55446/IJE.2021.30

Issue

Section

Research Articles

References

Abbott W S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265-267.

Ali S S, Asif M. 2011. Comparative field efficacy of dust formulation and liquid formulation of Steinernema seemae based biopesticide and other IPM options against Helicoverpa armigera (Hubner) Infesting Chickpea. Trends in Biosciences 4(1): 35-37.

Dhaliwal G S, Jindal V, Dhawan A K. 2010. Insect pest problems and crop losses: changing trends. Indian Journal of Ecology 37: 1-7.

Divya K, Sankar M, Marulasiddesha K N, Sambashiv R, Krupanidhi K. 2011. Formulation technology of entomopathogenic nematode for the control of the cotton boll worm, Helicoverpa armigera. Bioscience Discovery 2(2): 174-180.

Glazer I. 1997. Effects of infected insects on secondary invasion of steinernematid entomopathogenic nematodes. Parasitology 114: 597-604.

Grewal P S. 2000. Enhanced ambient storage stability of an entomopathogenic nematode through anhydrobiosis. Pest Management Science 56(5): 401-406.

Grewal P S. 2002. Formulation and Application Technology. In: Entomopathogenic nematology (ed.) R. Gaugler, CAB International, Wallingford, UK, 266-287 pp.

Hugar P S. 2010. Evaluation of EPN formulations, their shelf life and efficacy of Heterorhabditis indica (Heterorhabditidae: Nematoda) against economically important pests. Doctoral dissertation, UAS, Dharwad. p. 142.

Jagdale G B, Grewal P S. 2007. Storage temperature influences desiccation and ultra violet radiation tolerance of entomopathogenic nematodes. Journal of Thermal Biology 32(1): 20-27.

Navon A, Nagalakshmi V K, Shlomit L, Salame L. Glazer I. 2002. Effectiveness of entomopathogenic nematodes in an alginate gel formulation against lepidopterous pests. Biocontrol Science and Technology 12(6): 737-746.

Seenivasan N. 2017. Evaluation of different media for mass production of native strains of Heterorhabditis bacteriophora and Steinernema carpocapsae by in vitro solid culture. International Journal of Research Studies in Zoology 3(2): 45-50.

Seenivasan N. 2020. Virulence of native entomopathogenic nematodes to manage cotton insect pests Helicoverpa armigera, Earias vittella and Spodoptera litura. Journal of Cotton Research and Development 34(1): 84-91.

Seenivasan N, Sivakumar M. 2012. Bio-prospecting of naturally occurring entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) isolated from cotton fields at Tamil Nadu, India. Proceedings. 2nd International Symposium of Bio-Pesticides and Eco-toxicological Network (2nd IS-BioPEN): 24-26 September 2012, Bangkok, Thailand. p. 57.

Seenivasan N, Sivakumar M. 2014. Screening for environmental stress-tolerant entomopathogenic nematodes virulent against cotton bollworms. Phytoparasitica 42: 165-177.

Seenivasan N, Prabhu S, Makesh S, Sivakumar M. 2012. Natural occurrence of entomopathogenic nematode species (Rhabditida: Steinernematidae and Heterorhabditidae) in cotton fields of Tamil Nadu, India. Journal of Natural History 46: 2829-2843.

Umamaheswari R, Sivakumar M, Subramanian S. 2006. Survival and infectivity of entomopathogenic nematodes in alginate gel formulations against rice meal moth larva, Corcyra cephalonica Stainton. Natural Product Radiance 5: 95-98.