Effect of Dietary Azasteroids on the Growth and Development of the Gram Pod Borer Helicoverpa armigera (Hubner)

Authors

  • Rita Rath Department of Zoology, Dyal Singh College, University of Delhi, Delhi 110007, NCT Delhi
  • Ranjana Saxena Department of Zoology, Dyal Singh College, University of Delhi, Delhi 110007, NCT Delhi
  • Versha Goel Maitreyi College, University of Delhi, Delhi 110007, NCT Delhi
  • Renu Gupta Ramjas College, University of Delhi, Delhi 110007, NCT Delhi

DOI:

https://doi.org/10.55446/IJE.2022.180

Keywords:

Helicoverpa armigera, 25-azacholestane, 25-azacoprostane, sterol, cholesterol, larval duration, larval mortality, pupal duration, pupal mortality, adult emergence, metabolism, utilization, inhibition, IPM

Abstract

Effect of two azasteroids, 25-azacholestane and 25-azacoprostane was studied on growth and development of an economically important phytophagous pest, Helicoverpa armigera (Hubner) causing extensive damage to crops like cotton, pigeon pea, chickpea and others. 25-Azacholestane caused an increase in larval mortality (36% in control to 52% at 25 ppm), decrease in pupation (64% in control to 48% at 25 ppm) and decrease in adult emergence (60% in control to 28% at 25 ppm). Larval and pupal duration was significantly more as the azacholestane concentration increased to 25 ppm (p<0.001). Similar results were observed in case of 25-azacoprostane treatment also. Both the azasteroids were found to have inhibitory effect on the growth and development of H. armigera. Formation of larval-pupal intermediates and adults with abnormal wings was also observed. Addition of 0.1% cholesterol along with 10 ppm of 25-azacoprostane in the diet reversed the inhibitory effect of the azasteroid.

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Published

2022-03-24

How to Cite

Rath, R. ., Saxena, R., Goel, V., & Gupta, R. . (2022). Effect of Dietary Azasteroids on the Growth and Development of the Gram Pod Borer <i>Helicoverpa armigera</i> (Hubner). Indian Journal of Entomology, 85(1), 155–159. https://doi.org/10.55446/IJE.2022.180

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Section

Research Articles

References

Agale S V, Gupta R, Rangarao G V, Gopalakrishna S, Jagdish J, Wani S P. 2021. Efficacy of some selected biopesticides against Helicoverpa armigera (Hub) in pigeon pea under natural condition. Legume Research 44: 463-471.

Agarwal H C, Gupta R, Rath R, Goel V. 1990. Sterol inhibition in Chilo partellus. International Journal of Tropical Insect Science 11(4-5): 583-591.

Al-Izzi M A J, Hopkins T L. 1982. Effects of 25 azasteroids on development and reproduction of the southwestern corn borer, Diatraea grandiosella Dyar. Journal of Insect Physiology 28: 267-271.

Behmer S T, Elias D O, Grebenok R J. 1999. Phytosterol metabolism and absorption in the generalist grasshopper, Schistocerca americana. Archives of Insect Biochemistry and Physiology 42: 13-25.

Behmer S T, Nes W D. 2003. Insect sterol nutrition and physiology: An overview. Advances in Insect Physiology 31: 1-72.

Burbiel J, Bracher F. 2013. Azasteroids as antifungal. Steroids. 68 (7-8): 587-94.

Cheong M C, Na K, Kim H, Jeong S K, Joo H J, Chitwood D J, Paik Y K. 2011. A potential biochemical mechanism underlying the influence of sterol deprivation stress on Caenorhabditis elegans longevity. The Journal of Biological Chemistry 286: 7248-7256.

Chippendale G M, Reddy G P V. 1973. Hypocholestemic agents and developmental suppression of the southwestern corn borer. Journal of Economic Entomology 66: 1336-1337.

Choi B K, Chitwood D J, Paik Y K. 2003. Proteomic changes during disturbance of cholesterol metabolism by azacoprostane treatment in Caenorhabditis elegans. Molecular and Cellular Proteomics. 2: 1086-1095.

Darnet S, Martin L B B, Mercier P, Bracher F, Geoffroy P, Schaller H. 2020. Inhibition of phytosterol biosynthesis by azasteroids. NCBI Molecules 25 (5): 1111.

Earle N W, Lambremont E N, Burks M L, Slatten B H, Bennett A G. 1967. Conversion of beta sitosterol to cholesterol in the boll weevil and the inhibition of larval development by two azasteroids. Journal of Economic Entomology 60: 291-293.

Edosa T T. 2019. Review on bio-intensive management of African bollworm, Helicoverpa armigera (Hub): Botanical and semiochemical perspective. African Journal of Agricultural Research 14 (1): 1-9.

Entringer P F, Majerowicz D, Gondim K C. 2021. Fate of dietary cholesterol in the kissing bug, Rhodnius prolixus. Frontiers of Physiology 12: 654565.

Gilbert L I, Rybczynski R, Warren J T. 2002. Control and biochemical nature of ecdysteroidogenic pathway. Annual Review of Entomology 47: 883-916.

Goel V, Agarwal H C. 1987. Effect of 25-azacholestrol on development in Locusta migratoria ( Orthoptera: acrididae). Entomon 12 (2): 127-129.

Goel V, Rath R, Saxena R. 2021. Incorporation of [³H]-cholesterol in various tissues during ovarian development of Locusta migratoria L. (Orthoptera: Acrididae). International Journal of Entomological Research 6 (6): 17-22.

Haile F, Nowatzki T, Storer N. 2021. Overview of pest status, potential risk and management consideration of Helicoverpa armigera (Hubner) (Lep: Noctuidae) for US soybean production. Journal of Integrated Pest Management 12(1): 1-10.

Jing X, Behmer S T. 2020. Insect sterol nutrition: Physiological mechanisms, ecology and applications. Annual Review of Entomology 65: 251-271.

Kuthiala A, Agarwal H C, Thompson M J, Svoboda J A. 1987. 25-Azasteroid inhibition of development and conversion of C28 and C29 phytosterols to cholesterol in Spodoptera litura (F). Archives of Insect Biochemistry and Physiology 4: 57-66.

Li S, Jing X. 2020. Fates of dietary sterols in the insect alimentary canal. Current Opinion of Insect Science 41: 106-111.

Rath R. 1988. Utilization and metabolism of sterols and their inhibition in Heliothis armigera (Hubner). Ph D Thesis, University of Delhi.

Rath R, Agarwal H C. 1988. Growth of gram pod borer, Heliothis armigera (Hubner) in relation to dietary sterols. Indian Journal of Experimental Biology 26: 993-995.

Ravi K C, Mohan K S, Manjunath T M, Head G, Patil P V, Angeline Greba D P, Premalatha K, Peter J, Rao N G V. 2005. Relative abundance of Helicoverpa armigera (Lepidoptera; Noctuidae) on different host crops in India and the role of these crops as refuge for Bacillus thuringiensis cotton. Environmental Entomology 34 (1): 59-66.

Svoboda J A, Robbins W E. 1971. The inhibitive effects of azasterols on sterol metabolism and growth and development in insects with special reference to the tobacco hornworm. Lipids 6: 113-119.

Svoboda J A, Thompson M J, Robbins W E. 1972. Azasteroids, potent inhibitors of insect molting and metamorphosis. Lipids 7(8): 553-556.

Svoboda J A, Thompson M J. 1985. Steroids. Kerkut G A, Gilbert L I (eds). Comprehensive Insect Physiology, Biochemistry and Pharmacology. Pergamon Press, Oxford 10:137-175.

Svoboda J A, Herbert E W, Thompson M J. 1987. Effects of steroid metabolism inhibitors and ecdysteroid analogs on honey bee sterol metabolism and development. Archives of Insect Biochemistry and Physiology 6: 1-8.

Svoboda J A, Feldlaufer M F. 1991. Neutral sterol metabolism in insects. Lipids 26(8): 614-618.

Svoboda J A, Weirich G F. 1995. Sterol metabolism in tobacco hornworm, Manduca sexta- a review. Lipids 30 (3): 263-7.

Svoboda J A, Ross S A, Ness W D. 1995. Comparative studies of metabolism of 4- dimethyl, 4-monosodium methyl and 4, 4-dimethyl sterols in Manduca sexta. Lipids 30 (1): 91-94.

Tarlochan D S, Carlson G R, Dat P L. 1998. New ecdysteroids with ecdysteroidal and juvenile hormonal activity. Annual Review of Entomology 43: 545-569.

Thompson M J, Serban N N, Robbins W E, Svoboda J A, Shortino T J, Dutky S R, Cohen C F. 1975. Inhibitory effects of structurally modified azasteroids and related nitrogen containing steroids on insect growth and development. Lipids 1: 615-622.

Toprak U, Hegedus D, Dogan C, Guney G. 2020. A journey into the world of insect lipid metabolism. Archives of Insect Biochemistry and Physiology 104 (2): 21682.

Toprak U, Musselman, L P. 2021. From cellular biochemistry to systems physiology: New insights into insect lipid metabolism. Insect Biochemistry and Molecular Biology 133: 103585.

Walker W F, Svoboda J A. 1973. Suppression of Mexican bean beetle development by foliar application of azasterols and reversal by cholesterol. Entomologia Experimentalis et Applicata 16: 422-426.

Yang S, Kreutzberger A J B, Lee J, Kiessling V, Tamm L K. 2016. The role of cholesterol in membrane fusion. Chemistry and Physics of Lipids 199: 136-143